Characterization of LGALS3 (galectin-3) as a player in DNA damage response.
DNA damage repair (DDR) is an orchestrated process encompassing the injury detection to its complete resolution. DNA double-strand break lesions are repaired mainly by two distinct mechanisms: the error-free homologous recombination (HR) and the error-prone non-homologous end-joining. Galectin-3 (GAL3) is the unique member of the chimeric galectins subfamily and is ... reported to be involved in several cancer development and progression related events. Recently our group described a putative protein interaction between GAL3 and BARD1, the main partner of breast and ovarian cancer susceptibility gene product BRCA1, both involved in HR pathway. In this report we characterized GAL3/BARD1 protein interaction and evaluated the role of GAL3 in DDR pathways using GAL3 silenced human cells exposed to different DNA damage agents. In the absence of GAL3 we observed a delayed DDR response activation, as well as a decrease in the G 2/M cell cycle checkpoint arrest associated with HR pathway. Moreover, using a TAP-MS approach we also determined the protein interaction network of GAL3.
Mesh Terms:
Antineoplastic Agents, BRCA1 Protein, Carboplatin, DNA Damage, DNA Repair, Etoposide, G2 Phase Cell Cycle Checkpoints, Galectin 3, Gene Silencing, HEK293 Cells, HeLa Cells, Humans, Mitomycin, Protein Interaction Maps, Signal Transduction, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitination
Antineoplastic Agents, BRCA1 Protein, Carboplatin, DNA Damage, DNA Repair, Etoposide, G2 Phase Cell Cycle Checkpoints, Galectin 3, Gene Silencing, HEK293 Cells, HeLa Cells, Humans, Mitomycin, Protein Interaction Maps, Signal Transduction, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitination
Cancer Biol. Ther.
Date: Jul. 01, 2014
PubMed ID: 24755837
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