Evidence for a functional link between profilin and CAP in the yeast S. cerevisiae.

CAP is a component of the S. cerevisiae adenylyl cyclase complex. The N-terminal domain is required for cellular RAS responsiveness. Loss of the C-terminal domain is associated with morphological and nutritional defects. Here we report that cap- cells bud randomly and are defective in actin distribution. The morphological and nutritional ...
defects associated with loss of the CAP C-terminal domain are suppressed by over-expression of PFY, the gene encoding profilin, an actin- and polyphosphoinositide-binding protein. The phenotype of cells lacking PFY resembles that of cells lacking the CAP C-terminal domain. Study of mutated yeast profilins and profilins from Acanthamoeba suggests that the ability of profilin to suppress cap- cells is dependent upon a property other than, or in addition to, its ability to bind actin. This property may be its ability to bind polyphosphoinositides. We propose that CAP and profilin provide a link between growth signals and remodeling of the cellular cytoskeleton.
Mesh Terms:
Actins, Adenylate Cyclase, Amino Acid Sequence, Base Sequence, Cell Division, Cloning, Molecular, Contractile Proteins, Cytoskeleton, DNA Mutational Analysis, DNA, Fungal, Fungal Proteins, Genes, Fungal, Microfilament Proteins, Molecular Sequence Data, Oligonucleotides, Profilins, Restriction Mapping, Saccharomyces cerevisiae
Cell
Date: Aug. 09, 1991
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