Kaiso interacts with p120-catenin to regulate β-catenin expression at the transcriptional level.

We have reported that p120-catenin could regulate β-catenin transcription in lung cancer cells, but the specific mechanism is unclear.In this study, bisulfite sequencing PCR showed that the β-catenin promoter region in SPC-A-1 and LTEP-a-2 lung cancer cell lines has Kaiso binding sites sequences and CpG islands which may combine with ...
Kaiso. The demethylating reagent 5-Aza-2'-deoxycytidine significantly upregulated β-catenin mRNA expression in lung cancer cell lines, whereas expression was significantly reduced following transfection with Kaiso. However, the upregulation of β-catenin mRNA expression after treatment with 5-Aza-2'-deoxycytidine was not reduced by subsequent transfection with Kaiso cDNA. Chromatin immunoprecipitation showed that, in lung cancer cell lines, methylated CpG-dinucleotides sequences combined with Kaiso and the Kaiso binding sites sequence did not. The capacity of Kaiso to combine with p120-catenin isoforms was confirmed by immunoprecipitation.Based on these results, we concluded that Kaiso participates in the regulation by p120ctn of β-catenin mRNA expression in the lung cancer cell lines.
Mesh Terms:
Azacitidine, Blotting, Western, Catenins, Cell Line, Tumor, CpG Islands, DNA Methylation, Enzyme Inhibitors, Gene Expression Regulation, Neoplastic, Humans, Lung Neoplasms, Promoter Regions, Genetic, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, Transcription, Genetic, Up-Regulation, beta Catenin
PLoS ONE
Date: Feb. 06, 2014
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