Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription.

Five purified protein components, RNA polymerase I, Rrn3p, core factor, TBP (TATA-binding protein), and upstream activation factor, are sufficient for high level transcription in vitro from the Saccharomyces cerevisiae rDNA promoter. Rrn3p and pol I form a complex in solution that is active in specific initiation. Three protein components, pol ...
I, Rrn3p, and core factor, and promoter sequence to -38, suffice for basal transcription. Unlike pol II and pol III, yeast pol I basal transcription does not require TBP. Instead, TBP, upstream activation factor, and the upstream element of the promoter together stimulate pol I basal transcription to a fully activated level. The role of TBP in pol I transcription is fundamentally different from its role in pol II or pol III transcription.
Mesh Terms:
Amino Acid Sequence, DNA, Ribosomal, DNA-Binding Proteins, Fungal Proteins, Molecular Sequence Data, Pol1 Transcription Initiation Complex Proteins, Promoter Regions, Genetic, RNA Polymerase I, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA Box, TATA-Box Binding Protein, Transcription Factors, Transcription, Genetic
J. Biol. Chem.
Date: Dec. 11, 1998
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