SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required ... for ubiquitin-mediated proteolysis of Cin2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Binding Sites, Cell Cycle, Cell Cycle Proteins, Cyclins, F-Box Proteins, Fungal Proteins, G1 Phase, G2 Phase, Gene Dosage, Gene Expression, Humans, Ligases, Mitosis, Molecular Sequence Data, Molecular Structure, Mutation, Protein Structure, Tertiary, Proteins, S Phase, S-Phase Kinase-Associated Proteins, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins, Yeasts
Amino Acid Sequence, Base Sequence, Binding Sites, Cell Cycle, Cell Cycle Proteins, Cyclins, F-Box Proteins, Fungal Proteins, G1 Phase, G2 Phase, Gene Dosage, Gene Expression, Humans, Ligases, Mitosis, Molecular Sequence Data, Molecular Structure, Mutation, Protein Structure, Tertiary, Proteins, S Phase, S-Phase Kinase-Associated Proteins, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins, Yeasts
Cell
Date: Jul. 26, 1996
PubMed ID: 8706131
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