Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.
The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon ... the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Anti-GAL4 antisera were raised against a GAL4-URA3 fusion protein, which could be purified to homogeneity in a single step with the use of an affinity chromatographic procedure for the URA3 gene product.
Mesh Terms:
DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Galactose, Gene Expression Regulation, Immunologic Techniques, Macromolecular Substances, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors
DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Galactose, Gene Expression Regulation, Immunologic Techniques, Macromolecular Substances, Protein Binding, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Transcription Factors
Mol. Cell. Biol.
Date: Oct. 01, 1987
PubMed ID: 3316976
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