Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response.
Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, ... a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.
Mesh Terms:
Base Sequence, Blotting, Northern, DNA Fingerprinting, Down-Regulation, Galactose, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, Molecular Sequence Data, Plasmids, Promoter Regions, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Transcription, Genetic, Zinc Fingers
Base Sequence, Blotting, Northern, DNA Fingerprinting, Down-Regulation, Galactose, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Suppressor, Molecular Sequence Data, Plasmids, Promoter Regions, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Transcription, Genetic, Zinc Fingers
EMBO J.
Date: Nov. 01, 1991
PubMed ID: 1915298
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