A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a counterpart of the Drosophila melanogaster diphenol oxidase A2 gene, Dox-A2.
NIN1 is an essential gene for growth of the yeast Saccharomyces cerevisiae and was recently found to encode a component of the regulatory subunit of the 26S proteasome. The nin1-1 mutant is temperature sensitive and its main defect is in G1/S progression and G2/M progression at non-permissive temperatures. One of ... the two multicopy suppressors of nin1-1, SUN2 (SUppressor of Nin1-1), was found to encode a protein of 523 amino acids whose sequence is similar to those of Drosophila melanogaster diphenol oxidase A2 and the mouse mast-cell Tum(-) transplantation antigen, P91A. The C-terminal half of Sun2p was found to be functional as Sun2p at 25 degrees C, 30 degrees C, and 34 degrees C but not at 37 degrees C. The open reading frame (ORF) of the Drosophila diphenol oxidase A2 gene (Dox-A2) was obtained from a lambda phage cDNA library using the polymerase chain reaction technique. The Dox-A2 ORF driven by the TDH3 promoter complemented the phenotype of a strain deleted for sun2. This Dox-A2-dependent strain was temperature sensitive and accumulated dumb-bell-shaped cells, with an undivided nucleus at the isthmus, after temperature upshift. This morphology is similar to that of nin1-1 cells kept at a restrictive temperature. These results suggest that SUN2 is a functional counterpart of Dox-A2 and that these genes play a pivotal role in the cell cycle in each organism.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Catechol Oxidase, Cell Cycle Proteins, DNA, Fungal, Drosophila melanogaster, Fungal Proteins, Gene Expression Regulation, Fungal, Glyceraldehyde-3-Phosphate Dehydrogenases, Histocompatibility Antigens, Mice, Molecular Sequence Data, Open Reading Frames, Phenotype, Promoter Regions, Genetic, Proteasome Endopeptidase Complex, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic, Temperature
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Catechol Oxidase, Cell Cycle Proteins, DNA, Fungal, Drosophila melanogaster, Fungal Proteins, Gene Expression Regulation, Fungal, Glyceraldehyde-3-Phosphate Dehydrogenases, Histocompatibility Antigens, Mice, Molecular Sequence Data, Open Reading Frames, Phenotype, Promoter Regions, Genetic, Proteasome Endopeptidase Complex, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Suppression, Genetic, Temperature
Mol. Gen. Genet.
Date: May. 23, 1996
PubMed ID: 8668124
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