MIG1 overexpression causes flocculation in Saccharomyces cerevisiae.
MIG1, encoding a C2H2 zinc-finger repressor protein involved in carbon catabolite repression, was found to play a role in non-sexual flocculation of Saccharomyces cerevisiae. Disruption of MIG1 in a flocculent mutant strain of NCYC 227, resulted in a non-flocculent phenotype. Expression of MIG1 on a 2 mu pRS426 vector in ... a non-flocculent strain, YM 4134, caused flocculation; MIG1 on a high-copy-number LEU2-d plasmid caused intense flocculation in the same strain. Mutations in the SSN6 and TUP1 genes confer a flocculent phenotype in non-flocculent strains of S. cerevisiae, and it has been shown that Mig1 can tether the Ssn6p-Tup1p complex to the regulatory regions of glucose-repressible genes. Mutations in tup1 in a MIG1 background caused flocculation while double mutants of TUP1 and MIG1 did not flocculate. Based on these results, a model for the role of MIG1 in flocculation gene regulation is proposed.
Mesh Terms:
Blotting, Northern, Cloning, Molecular, DNA-Binding Proteins, Flocculation, Flocculation Tests, Gene Expression Regulation, Fungal, Mutagenesis, Mutagenesis, Insertional, Plasmids, RNA, Messenger, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Blotting, Northern, Cloning, Molecular, DNA-Binding Proteins, Flocculation, Flocculation Tests, Gene Expression Regulation, Fungal, Mutagenesis, Mutagenesis, Insertional, Plasmids, RNA, Messenger, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Microbiology (Reading, Engl.)
Date: Sep. 01, 1996
PubMed ID: 8828236
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