Global role for chromatin remodeling enzymes in mitotic gene expression.

Regulation of eukaryotic gene expression requires ATP-dependent chromatin remodeling enzymes, such as SWI/SNF, and histone acetyltransferases, such as Gcn5p. Here we show that SWI/SNF remodeling controls recruitment of Gcn5p HAT activity to many genes in late mitosis and that these chromatin remodeling enzymes play a role in regulating mitotic exit. ...
In contrast, interphase expression of GAL1, HIS3, PHO5, and PHO8 is accompanied by SWI/SNF-independent recruitment of Gcn5p HAT activity. Surprisingly, prearresting cells in late mitosis imposes a requirement for SWI/SNF in recruiting Gcn5p HAT activity to the GAL1 promoter, and GAL1 expression also becomes dependent on both chromatin remodeling enzymes. We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin.
Mesh Terms:
Acetylation, Acetyltransferases, Anaphase, Cell Cycle Proteins, Chromatin, DNA-Binding Proteins, Fungal Proteins, Gene Deletion, Gene Expression Regulation, Fungal, Genes, Fungal, Histone Acetyltransferases, Histones, Interphase, Mitosis, Nocodazole, Nucleic Acid Conformation, Phenotype, Promoter Regions, Genetic, Protein Kinases, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors, Transcription Factors
Cell
Date: Sep. 01, 2000
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