Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines.
In this study, we isolated and characterized a human cyclin A-like gene that we named cyclin A1. Cyclin A1 has 48% identity with human cyclin A and is more related to the recently cloned murine cyclin A1 (84% identity). The human cyclin A1 is specifically expressed in testis and brain ... among all of the normal tissues that we studied by Northern blot analysis; in addition, it is expressed in several myeloid leukemia cell lines, including ML-1, U937, NB4, KG-1, and THP1. A sensitive reverse transcription-PCR-Southern blot method also detected low-level expression of this gene in many other hematopoietic and nonhematopoietic cell lines. The expression of cyclin A1 mRNA is differentiation- and cell cycle-regulated in the ML-1 cells. We raised polyclonal antibodies against a glutathione S-transferase-cyclin A1 fusion protein produced in Escherichia coli. In immunoblot analyses, the antibodies recognized the Mr 65,000 cyclin A1 protein in ML-1 cells. The anti-cyclin A1 also immunoprecipitated the Mr 65,000 cyclin A1, along with the Mr 33,000 cyclin-dependent kinase (CDK) 2 and other proteins at Mr 39,000, 42,000, 45,000, 95,000, and 110,000. In an in vitro kinase assay, the CDK2-cyclin A1 complex precipitated by anti-cyclin A1 showed kinase activities against histone H1. In a yeast two-hybrid assay, cyclin A1 can bind to CDK2 but not to CDC2, CDK4, and CDK5. We mapped the human cyclin A1 gene to chromosome 13q12.3-q13, approximately 1000 kb from the sequence-tagged site marker WI-3374.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cell Differentiation, Chromosomes, Human, Pair 13, Cloning, Molecular, Cyclin A, Cyclin A1, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, Cyclins, Gene Expression Regulation, Neoplastic, Genes, Hematopoiesis, Humans, Leukemia, Myeloid, Male, Mice, Molecular Sequence Data, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, RNA, Messenger, Sequence Alignment, Sequence Homology, Amino Acid, Testis, Xenopus Proteins, Xenopus laevis
Amino Acid Sequence, Animals, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cell Differentiation, Chromosomes, Human, Pair 13, Cloning, Molecular, Cyclin A, Cyclin A1, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinases, Cyclins, Gene Expression Regulation, Neoplastic, Genes, Hematopoiesis, Humans, Leukemia, Myeloid, Male, Mice, Molecular Sequence Data, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, RNA, Messenger, Sequence Alignment, Sequence Homology, Amino Acid, Testis, Xenopus Proteins, Xenopus laevis
Cancer Res.
Date: Mar. 01, 1997
PubMed ID: 9041194
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