p38-Mediated phosphorylation of Eps15 endocytic adaptor protein.
Epidermal growth factor receptor pathway substrate 15 (Eps15) has been suggested to be involved in the endocytosis of cell surface receptors, including epidermal growth factor receptor (EGFR). Eps15 is phosphorylated at Tyr-849 upon stimulation with EGF during endocytic processes. In the present study, we found that stimulation of HeLa cells ... with EGF or TNF-α induced transient phosphorylation of Eps15 at Ser-796. Inhibition of p38 completely blocked phosphorylation and recombinant p38α directly phosphorylated the residue. These results demonstrate a novel stress kinase-mediated signaling pathway to Eps15 endocytic adapter protein.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Cell Line, Tumor, Chromatography, Liquid, Enzyme Inhibitors, Epidermal Growth Factor, HEK293 Cells, HeLa Cells, Humans, Imidazoles, Immunoblotting, Nanotechnology, Phosphorylation, Pyridines, RNA Interference, Serine, Signal Transduction, Tandem Mass Spectrometry, Tumor Necrosis Factor-alpha, p38 Mitogen-Activated Protein Kinases
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Cell Line, Tumor, Chromatography, Liquid, Enzyme Inhibitors, Epidermal Growth Factor, HEK293 Cells, HeLa Cells, Humans, Imidazoles, Immunoblotting, Nanotechnology, Phosphorylation, Pyridines, RNA Interference, Serine, Signal Transduction, Tandem Mass Spectrometry, Tumor Necrosis Factor-alpha, p38 Mitogen-Activated Protein Kinases
FEBS Lett.
Date: Jan. 03, 2014
PubMed ID: 24269888
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