Pokemon (FBI-1) interacts with Smad4 to repress TGF-β-induced transcriptional responses.
Pokemon, an important proto-oncoprotein, is a transcriptional repressor that belongs to the POK (POZ and Krueppel) family. Smad4, a key component of TGF-β pathway, plays an essential role in TGF-β-induced transcriptional responses. In this study, we show that Pokemon can interact directly with Smad4 both in vitro and in vivo. ... Overexpression of Pokemon decreases TGF-β-induced transcriptional activities, whereas knockdown of Pokemon increases these activities. Interestingly, Pokemon does not affect activation of Smad2/3, formation of Smads complex, or DNA binding activity of Smad4. TGF-β1 treatment increases the interaction between Pokemon and Smad4, and also enhances the recruitment of Pokemon to Smad4-DNA complex. In addition, we also find that Pokemon recruits HDAC1 to Smad4 complex but decreases the interaction between Smad4 and p300/CBP. Taken together, all these data suggest that Pokemon is a new partner of Smad4 and plays a negative role in TGF-β pathway.
Mesh Terms:
Binding Sites, DNA-Binding Proteins, Hep G2 Cells, Humans, Protein Interaction Maps, Signal Transduction, Smad2 Protein, Smad3 Protein, Smad4 Protein, Transcription Factors, Transcriptional Activation, Transforming Growth Factor beta
Binding Sites, DNA-Binding Proteins, Hep G2 Cells, Humans, Protein Interaction Maps, Signal Transduction, Smad2 Protein, Smad3 Protein, Smad4 Protein, Transcription Factors, Transcriptional Activation, Transforming Growth Factor beta
Biochim. Biophys. Acta
Date: Mar. 01, 2015
PubMed ID: 25514493
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