Xu X, Liu J, Shen C, Ding L, Zhong F, Ouyang Y, Wang Y, He S

Cell adhesion-mediated drug resistance (CAM-DR) is one of the mechanisms underlying the drug resistance in multiple myeloma (MM). Ubiquitin-specific protease 14 (USP14) is down-regulated in the apoptotic model and up-regulated in the adhesive model of MM. The present study was undertaken to determine the role of USP14 in CAM-DR of ...

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MM cells.We examined the expression of USP14 in the apoptotic model of MM. The mechanism of USP14 in the process of apoptosis was further explored by flow cytometry assay and co-immunoprecipitation. We then performed the cell co-culture and adhesion assay and cell viability assay to investigate the effect of USP14 on adhesive rate and drug resistance in MM.We discovered that USP14 played a negative role in cell apoptosis, which is correlated with Bcl-xl. Moreover, over-expression of USP14 in MM cell adhesion model could enhance the ability of cell adhesion by regulating Wnt signaling pathways, thereby promoting the CAM-DR in MM.USP14 participates in CAM-DR of MM through acting as a bridge between Bcl-xl apoptotic pathway and Wnt-signaling pathways and may be represented as a good candidate for pursuing clinical trials in MM. This article is protected by copyright. All rights reserved.

Date: Dec. 29, 2015

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