A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae.
Rad53 and Mec1 are protein kinases required for DNA replication and recovery from DNA damage in Saccharomyces cerevisiae. Here, we show that rad53, but not mec1 mutants, are extremely sensitive to histone overexpression, as Rad53 is required for degradation of excess histones. Consequently, excess histones accumulate in rad53 mutants, resulting ... in slow growth, DNA damage sensitivity, and chromosome loss phenotypes that are significantly suppressed by a reduction in histone gene dosage. Rad53 monitors excess histones by associating with them in a dynamic complex that is modulated by its kinase activity. Our results argue that Rad53 contributes to genome stability independently of Mec1 by preventing the damaging effects of excess histones both during normal cell cycle progression and in response to DNA damage.
Mesh Terms:
Cell Cycle Proteins, Cells, Cultured, DNA Damage, Gene Dosage, Gene Expression Regulation, Fungal, Genomic Instability, Histones, Macromolecular Substances, Mutation, Protein Binding, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Up-Regulation
Cell Cycle Proteins, Cells, Cultured, DNA Damage, Gene Dosage, Gene Expression Regulation, Fungal, Genomic Instability, Histones, Macromolecular Substances, Mutation, Protein Binding, Protein-Serine-Threonine Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Up-Regulation
Cell
Date: Nov. 26, 2003
PubMed ID: 14651846
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