The seventh zinc finger motif of A20 is required for the suppression of TNF-α-induced apoptosis.
The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seventh zinc finger motif (ZnF7), the involvement of ZnF7 in TNF-α-induced apoptosis ... is not clear. In this study, ZnF7 was found to be important for A20-mediated suppression of TNF-α-induced apoptosis. We also found that the ubiquitin ligases cIAP1/2 are required for A20 to suppress TNF-α-induced apoptosis. Because A20 binds to cIAP1/2 through ZnF7, these results suggest that A20 may control cIAP1/2 when suppressing TNF-α-induced apoptosis.
Mesh Terms:
Amino Acid Substitution, Animals, Apoptosis, Cell Line, Cell Survival, Cysteine Endopeptidases, DNA-Binding Proteins, Enzyme Inhibitors, Humans, Inhibitor of Apoptosis Proteins, Interleukin-1alpha, Intracellular Signaling Peptides and Proteins, Mice, Mutant Proteins, NF-kappa B, Nuclear Proteins, Recombinant Proteins, Tumor Necrosis Factor-alpha, Ubiquitin-Protein Ligases, Zinc Fingers
Amino Acid Substitution, Animals, Apoptosis, Cell Line, Cell Survival, Cysteine Endopeptidases, DNA-Binding Proteins, Enzyme Inhibitors, Humans, Inhibitor of Apoptosis Proteins, Interleukin-1alpha, Intracellular Signaling Peptides and Proteins, Mice, Mutant Proteins, NF-kappa B, Nuclear Proteins, Recombinant Proteins, Tumor Necrosis Factor-alpha, Ubiquitin-Protein Ligases, Zinc Fingers
FEBS Lett.
Date: May. 22, 2015
PubMed ID: 25911380
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