Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.

It was recently demonstrated that MCPIP1 is a critical factor that controls inflammation and immune homeostasis; however, the relationship between MCPIP1 and other members of this protein family is largely unknown. Here, we report that MCPIP1 interacts with MCPIP4 to form a protein complex, but acts independently in the regulation ...
of IL-6 mRNA degradation. In an effort to identify MCPIP1-interacting proteins by co-immunoprecipitation (Co-IP) and mass-spec analysis, MCPIP4 was identified as a MCPIP1-interacting protein, which was further confirmed by Co-IP and mammalian two-hybrid assay. Immunofluorescence staining showed that MCPIP4 was co-localized with MCPIP1 in the GW-body, which features GW182 and Argonaute 2. Further studies showed that MCPIP1 and MCPIP4 act independently in regulation of IL-6 mRNA degradation. These results suggest that MCPIP1 and MCPIP4 may additively contribute to control IL-6 production in vivo.
Mesh Terms:
Amino Acid Sequence, Animals, COS Cells, Cell Line, Cercopithecus aethiops, Gene Expression Regulation, HEK293 Cells, HeLa Cells, Humans, Immunoprecipitation, Interleukin-6, Macrophages, Mice, Molecular Sequence Data, Plasmids, Protein Binding, Proteins, RNA Stability, RNA, Messenger, Recombinant Proteins, Ribonucleases, Signal Transduction, Transcription Factors, Two-Hybrid System Techniques
J. Biol. Chem.
Date: Aug. 21, 2015
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