A mechanism for the suppression of homologous recombination in G1 cells.
DNA repair by homologous recombination is highly suppressed in G1 cells to ensure that mitotic recombination occurs solely between sister chromatids. Although many homologous recombination factors are cell-cycle regulated, the identity of the events that are both necessary and sufficient to suppress recombination in G1 cells is unknown. Here we ... report that the cell cycle controls the interaction of BRCA1 with PALB2-BRCA2 to constrain BRCA2 function to the S/G2 phases in human cells. We found that the BRCA1-interaction site on PALB2 is targeted by an E3 ubiquitin ligase composed of KEAP1, a PALB2-interacting protein, in complex with cullin-3 (CUL3)-RBX1 (ref. 6). PALB2 ubiquitylation suppresses its interaction with BRCA1 and is counteracted by the deubiquitylase USP11, which is itself under cell cycle control. Restoration of the BRCA1-PALB2 interaction combined with the activation of DNA-end resection is sufficient to induce homologous recombination in G1, as measured by RAD51 recruitment, unscheduled DNA synthesis and a CRISPR-Cas9-based gene-targeting assay. We conclude that the mechanism prohibiting homologous recombination in G1 minimally consists of the suppression of DNA-end resection coupled with a multi-step block of the recruitment of BRCA2 to DNA damage sites that involves the inhibition of BRCA1-PALB2-BRCA2 complex assembly. We speculate that the ability to induce homologous recombination in G1 cells with defined factors could spur the development of gene-targeting applications in non-dividing cells.
Mesh Terms:
Amino Acid Sequence, BRCA1 Protein, BRCA2 Protein, CRISPR-Cas Systems, Carrier Proteins, Cell Line, Cullin Proteins, DNA, DNA Damage, DNA Repair, G1 Phase, G2 Phase, Gene Targeting, Homologous Recombination, Humans, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Multiprotein Complexes, Nuclear Proteins, Protein Binding, Rad51 Recombinase, S Phase, Thiolester Hydrolases, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitination
Amino Acid Sequence, BRCA1 Protein, BRCA2 Protein, CRISPR-Cas Systems, Carrier Proteins, Cell Line, Cullin Proteins, DNA, DNA Damage, DNA Repair, G1 Phase, G2 Phase, Gene Targeting, Homologous Recombination, Humans, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Multiprotein Complexes, Nuclear Proteins, Protein Binding, Rad51 Recombinase, S Phase, Thiolester Hydrolases, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Ubiquitination
Nature
Date: Dec. 17, 2015
PubMed ID: 26649820
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