The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
The cyclin-dependent kinase Cdk2 associates with cyclins A, D, and E and has been implicated in the control of the G1 to S phase transition in mammals. To identify potential Cdk2 regulators, we have employed an improved two-hybrid system to isolate human genes encoding Cdk-interacting proteins (Cips). CIP1 encodes a ... novel 21 kd protein that is found in cyclin A, cyclin D1, cyclin E, and Cdk2 immunoprecipitates. p21CIP1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk2, cyclin E-Cdk2, cyclin D1-Cdk4, and cyclin D2-Cdk4 complexes. Cotransfection experiments indicate that CIP1 and SV40 T antigen function in a mutually antagonistic manner to control cell cycle progression.
Mesh Terms:
Amino Acid Sequence, Antigens, Polyomavirus Transforming, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cloning, Molecular, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase Inhibitor p21, Cyclin-Dependent Kinases, Cyclins, Humans, Molecular Sequence Data, Phosphorylation, Protein Binding, Protein Kinase Inhibitors, Protein-Serine-Threonine Kinases, Recombinant Proteins, Retinoblastoma Protein
Amino Acid Sequence, Antigens, Polyomavirus Transforming, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cloning, Molecular, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase Inhibitor p21, Cyclin-Dependent Kinases, Cyclins, Humans, Molecular Sequence Data, Phosphorylation, Protein Binding, Protein Kinase Inhibitors, Protein-Serine-Threonine Kinases, Recombinant Proteins, Retinoblastoma Protein
Cell
Date: Nov. 19, 1993
PubMed ID: 8242751
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