Isolation and characterization of KIUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae.

The Kluyveromyces lactis UBP2 gene was isolated as a suppressor of a temperature-sensitive mutation in CBF2, a gene coding for a centromere-binding protein of Saccharomyces cerevisiae. The UBP genes are hydrolases than can cleave a ubiquitin moiety from a protein substrate. KlUBP2 is not essential for growth since a disruption ...
of the KlUBP2 gene had little effect, except for a slight decrease in the growth rate. The stability of centromere-containing plasmids was not influenced either. In addition to KlUBP2, five S. cerevisiae genes involved in the ubiquitination pathway could suppress the ts-mutation in the CBF2 gene, namely UBA1, UBA2, UBP1, UBP2 and YUH1, although YUH1 was the only one that could do this like KlUBP2 from a single-copy plasmid. Surprisingly, these genes encode proteins with antagonistic activity as two, UBA1 and UBA2, are ubiquitin-activating enzymes whereas the other three are de-ubiquitinating hydrolases.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Centromere, Conserved Sequence, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Kinetochores, Kluyveromyces, Ligases, Molecular Sequence Data, Mutation, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Suppression, Genetic, Ubiquitin-Activating Enzymes, Ubiquitin-Protein Ligases, Ubiquitins
Curr. Genet.
Date: Jul. 01, 2000
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