A novel mutation occurring in the PHO80 gene suppresses the PHO4c mutations of Saccharomyces cerevisiae.
We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isoleucine for ... methionine 42 of the Pho80 protein. Substitution of valine (PHO80-3) or leucine (PHO80-4) for the same methionine by site-directed mutagenesis also suppressed PHOc. Suppression by PHO80-2) did show some allele specificity. From these results we were able to delimit the region of PHo80 which may interact with the Pho4 protein.
Mesh Terms:
Alleles, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cyclins, DNA, Fungal, Fungal Proteins, Genes, Dominant, Genes, Fungal, Genes, Regulator, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Repressor Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic
Alleles, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cyclins, DNA, Fungal, Fungal Proteins, Genes, Dominant, Genes, Fungal, Genes, Regulator, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Repressor Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic
Curr. Genet.
Date: Feb. 01, 1992
PubMed ID: 1568260
View in: Pubmed Google Scholar
Download Curated Data For This Publication
19316
Switch View:
- Interactions 1