RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-κB pathway.

The RNA-binding protein RC3H1 (also known as ROQUIN) promotes TNFα mRNA decay via a 3'UTR constitutive decay element (CDE). Here we applied PAR-CLIP to human RC3H1 to identify ∼ 3,800 mRNA targets with >16,000 binding sites. A large number of sites are distinct from the consensus CDE and revealed a ...
structure-sequence motif with U-rich sequences embedded in hairpins. RC3H1 binds preferentially short-lived and DNA damage-induced mRNAs, indicating a role of this RNA-binding protein in the post-transcriptional regulation of the DNA damage response. Intriguingly, RC3H1 affects expression of the NF-κB pathway regulators such as IκBα and A20. RC3H1 uses ROQ and Zn-finger domains to contact a binding site in the A20 3'UTR, demonstrating a not yet recognized mode of RC3H1 binding. Knockdown of RC3H1 resulted in increased A20 protein expression, thereby interfering with IκB kinase and NF-κB activities, demonstrating that RC3H1 can modulate the activity of the IKK/NF-κB pathway.
Mesh Terms:
Binding Sites, Blotting, Western, DNA Damage, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Gene Knockdown Techniques, HEK293 Cells, Humans, I-kappa B Proteins, Intracellular Signaling Peptides and Proteins, NF-kappa B, Nuclear Proteins, RNA Processing, Post-Transcriptional, RNA Stability, RNA, Messenger, RNA-Binding Proteins, Signal Transduction, Ubiquitin-Protein Ligases
Nat Commun
Date: Jul. 15, 2015
Download Curated Data For This Publication
193617
Switch View:
  • Interactions 157