A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.
We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which the first step of mRNA splicing is inhibited. The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28). The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family ... of proteins that are thought to function as ATP-dependent RNA helicases. The cold sensitivity is caused by a glycine-to-glutamic acid change in a conserved sequence motif. Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable. In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5. These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.
Mesh Terms:
Amino Acid Sequence, Cold Temperature, Crosses, Genetic, Exons, Genes, Fungal, Genes, Suppressor, Kinetics, Models, Genetic, Molecular Sequence Data, Multigene Family, Mutation, Oligonucleotide Probes, RNA Helicases, RNA Nucleotidyltransferases, RNA Splicing, RNA, Messenger, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid
Amino Acid Sequence, Cold Temperature, Crosses, Genetic, Exons, Genes, Fungal, Genes, Suppressor, Kinetics, Models, Genetic, Molecular Sequence Data, Multigene Family, Mutation, Oligonucleotide Probes, RNA Helicases, RNA Nucleotidyltransferases, RNA Splicing, RNA, Messenger, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid
Genes Dev.
Date: Apr. 01, 1991
PubMed ID: 2010088
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