Boeck R, Lapeyre B, Brown CE, Sachs AB

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.

mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.

Mesh Terms:
Amino Acid Sequence, Fungal Proteins, Gene Deletion, Genotype, Kinetics, Molecular Sequence Data, Open Reading Frames, Plasmids, Poly(A)-Binding Proteins, RNA Cap-Binding Proteins, RNA Caps, RNA, Fungal, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Suppression, Genetic

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