Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.
Mesh Terms:
Amino Acid Sequence, Fungal Proteins, Gene Deletion, Genotype, Kinetics, Molecular Sequence Data, Open Reading Frames, Plasmids, Poly(A)-Binding Proteins, RNA Cap-Binding Proteins, RNA Caps, RNA, Fungal, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Suppression, Genetic
Mol. Cell. Biol. Sep. 01, 1998; 18(9);5062-72 [PUBMED:9710590]
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