Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated.
Poly(ADP-ribose) polymerase-1 (PARP-1) is activated by DNA strand breaks during cellular genotoxic stress response and catalyzes poly(ADP-ribosyl)ation of acceptor proteins. These acceptor proteins include those involved in modulation of chromatin structure, DNA synthesis, DNA repair, transcription, and cell cycle control. Thus, PARP-1 is believed to play a pivotal role in ... maintaining genome integrity through modulation of protein-protein and protein-DNA interactions. We previously described the association of PARP-1 with normal mammalian centromeres and human neocentromeres by affinity purification and immunofluorescence. Here we investigated the interaction of this protein with, and poly(ADP-ribosyl)ation of, three constitutive centromere proteins, Cenpa, Cenpb, and Cenpc, and a spindle checkpoint protein, Bub3. Immunoprecipitation and Western blot analyses demonstrate that Cenpa, Cenpb, and Bub3, but not Cenpc, interacted with PARP-1, and are poly(ADP-ribosyl)ated following induction of DNA damage. The results suggest a role of PARP-1 in centromere assembly/disassembly and checkpoint control. Demonstration of PARP-1-binding and poly(ADP-ribosyl)ation in three of the four proteins tested further suggests that many more centromere proteins may behave similarly and implicates PARP-1 as an important regulator of diverse centromere function.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, Animals, Autoantigens, Binding Sites, Blotting, Western, Cell Cycle Proteins, Cell Line, Cell Nucleus, Centromere, Centromere Protein B, Chromosomal Proteins, Non-Histone, DNA Repair, DNA-Binding Proteins, Mice, Molecular Sequence Data, Phenotype, Phosphorylation, Poly Adenosine Diphosphate Ribose, Poly(ADP-ribose) Polymerases, Precipitin Tests, Protein Binding, Transcription, Genetic
Amino Acid Motifs, Amino Acid Sequence, Animals, Autoantigens, Binding Sites, Blotting, Western, Cell Cycle Proteins, Cell Line, Cell Nucleus, Centromere, Centromere Protein B, Chromosomal Proteins, Non-Histone, DNA Repair, DNA-Binding Proteins, Mice, Molecular Sequence Data, Phenotype, Phosphorylation, Poly Adenosine Diphosphate Ribose, Poly(ADP-ribose) Polymerases, Precipitin Tests, Protein Binding, Transcription, Genetic
J. Biol. Chem.
Date: Jul. 26, 2002
PubMed ID: 12011073
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