Saccharomyces cerevisiae contains two functional citrate synthase genes.
The tricarboxylic acid cycle occurs within the mitochondria of the yeast Saccharomyces cerevisiae. A nuclear gene encoding the tricarboxylic acid cycle enzyme citrate synthase has previously been isolated (M. Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the ... isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2). Disruption of both genes in the yeast genome was necessary to produce classical citrate synthase-deficient phenotypes: glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source. Therefore, the citrate synthase produced from either gene was sufficient for these metabolic roles. Transcription of both genes was maximally repressed in medium containing both glucose and glutamate. However, transcription of CIT1 but not of CIT2 was derepressed in medium containing a nonfermentable carbon source. The significance of the presence of two genes encoding citrate synthase in S. cerevisiae is discussed.
Mesh Terms:
Chromosome Mapping, Citrate (si)-Synthase, DNA Restriction Enzymes, Gene Expression Regulation, Genes, Fungal, Genetic Complementation Test, Glucose, Glutamates, Lactates, Oxo-Acid-Lyases, RNA, Messenger, Saccharomyces cerevisiae, Transcription, Genetic
Chromosome Mapping, Citrate (si)-Synthase, DNA Restriction Enzymes, Gene Expression Regulation, Genes, Fungal, Genetic Complementation Test, Glucose, Glutamates, Lactates, Oxo-Acid-Lyases, RNA, Messenger, Saccharomyces cerevisiae, Transcription, Genetic
Mol. Cell. Biol.
Date: Jun. 01, 1986
PubMed ID: 3023912
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