Functional interaction of tumor suppressor DLC1 and caveolin-1 in cancer cells.

Deleted in liver cancer 1 (DLC1), a tumor suppressor gene frequently inactivated in non-small cell lung cancer (NSCLC) and other malignancies, encodes a multidomain protein with a RhoGTPase-activating (RhoGAP) domain and a StAR-related lipid transfer (START) domain. However, no interacting macromolecule has been mapped to the DLC1 START domain. Caveolin-1 ...
(CAV-1) functions as a tumor suppressor in most contexts and forms a complex with DLC1. Here, we have mapped the region of DLC1 required for interaction with CAV-1 to the DLC1 START domain. Mutation of the DLC1 START domain disrupted the interaction and colocalization with CAV-1. Moreover, DLC1 with a START domain mutation failed to suppress neoplastic growth, although it negatively regulated active Rho. CAV-1 and DLC1 expression levels were correlated in two public datasets of NSCLC lines and in two independent publicly available mRNA expression datasets of NSCLC tumors. Clinically, low DLC1 expression predicted a poor clinical outcome in patients with lung cancer. Together, our findings indicate that complex formation between the DLC1 START domain and CAV-1 contributes to DLC1 tumor suppression via a RhoGAP-independent mechanism, and suggest that DLC1 inactivation probably contributes to cancer progression.
Mesh Terms:
Animals, Caveolin 1, Cell Line, Tumor, Cell Movement, Cell Proliferation, Female, GTPase-Activating Proteins, Gene Deletion, Gene Expression Regulation, Neoplastic, Humans, Lung Neoplasms, Mice, Mice, Nude, Neoplasms, Prognosis, Protein Binding, Protein Interaction Domains and Motifs, Protein Transport, Tumor Suppressor Proteins
Cancer Res.
Date: Sep. 01, 2012
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