An interaction between p21ras and heat shock protein hsp60, a chaperonin.
Ras proteins play a crucial role in the development of neoplasia and in signal transduction in normal cells. In a search for proteins interacting with p21ras, we previously identified a protein of 60 kDa (p60) through use of a chemical cross-linker. Using information from partial amino acid sequencing of the ... purified protein, we isolated full-length cDNA clones encoding this 60-kDa protein. Nucleotide sequence analysis revealed that p60 is the murine heat shock protein hsp60, a chaperonin. Association of hsp60 with p21ras appears physiological, as the amount of hsp60 complexed to p21ras was similar even in cells over-expressing p21ras, and reversing the order of cross-linking and lysis of the cells, which releases large amounts of hsp60 from mitochondria, did not alter the amount of hsp60 cross-linked to p21ras.
Mesh Terms:
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Base Sequence, Cell Line, Chaperonins, Cloning, Molecular, Cross-Linking Reagents, Heat-Shock Proteins, Humans, Molecular Sequence Data, Oligodeoxyribonucleotides, Proteins, Proto-Oncogene Proteins p21(ras), Rats, Sequence Homology, Nucleic Acid, Succinimides
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Base Sequence, Cell Line, Chaperonins, Cloning, Molecular, Cross-Linking Reagents, Heat-Shock Proteins, Humans, Molecular Sequence Data, Oligodeoxyribonucleotides, Proteins, Proto-Oncogene Proteins p21(ras), Rats, Sequence Homology, Nucleic Acid, Succinimides
Proc. Natl. Acad. Sci. U.S.A.
Date: Mar. 15, 1992
PubMed ID: 1347942
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