The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins.
In yeast, certain resident trans-Golgi network (TGN) proteins achieve steady-state localization by cycling through late endosomes. Here, we show that chitin synthase III (Chs3p), an enzyme involved in the assembly of the cell wall at the mother-bud junction, populates an intracellular reservoir that is maintained by a cycle of transport ... between the TGN and early endosomes. Traffic of Chs3p from the TGN/early endosome to the cell surface requires CHS5 and CHS6, mutant alleles of which trap Chs3p in the TGN/early endosome. Disruption of the clathrin adaptor protein complex 1 (AP-1) restores Chs3p transport to the plasma membrane. Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN.
Mesh Terms:
Adaptor Proteins, Vesicular Transport, Carrier Proteins, Chitin Synthase, Endosomes, Fungal Proteins, Gene Expression Regulation, Fungal, Membrane Proteins, Mutation, Protein Transport, Qa-SNARE Proteins, Saccharomyces cerevisiae, Secretory Vesicles, trans-Golgi Network
Adaptor Proteins, Vesicular Transport, Carrier Proteins, Chitin Synthase, Endosomes, Fungal Proteins, Gene Expression Regulation, Fungal, Membrane Proteins, Mutation, Protein Transport, Qa-SNARE Proteins, Saccharomyces cerevisiae, Secretory Vesicles, trans-Golgi Network
Dev. Cell
Date: Mar. 01, 2002
PubMed ID: 11879634
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