p53 proteins accumulated by heat stress associate with heat shock proteins HSP72/HSC73 in human glioblastoma cell lines.
We investigated the accumulation of p53 proteins after heat stress and their association with HSP72/HSC73 using four human glioblastoma cell lines. Human glioblastoma cell lines U-87MG and A-172 exhibited no mutation in the region between the 2nd and 11th exons of the p53 gene, whereas A-7 and T98G had mutations ... in exon 5 and exon 7 of the p53 gene, respectively. In U-87MG and A-172, the levels of wild-type p53 protein were slightly increased by heat stress. Levels of mutant p53 protein were apparently increased by heat stress in A-7, but not in T98G. Furthermore, wild-type p53 proteins in both U-87MG and A-172 co-immunoprecipitated with anti-HSP72/HSC73 antibody and HSP72 and HSC73 in them co-immunoprecipitated with anti-p53 antibody as did the mutant p53 proteins. These findings suggest that p53 proteins accumulated by heat stress are associated with HSP72 and HSC73.
Mesh Terms:
Blotting, Western, DNA, Neoplasm, Exons, Genes, p53, Glioblastoma, HSP70 Heat-Shock Proteins, Hot Temperature, Humans, Mutation, Polymerase Chain Reaction, Precipitin Tests, Tumor Cells, Cultured, Tumor Suppressor Protein p53
Blotting, Western, DNA, Neoplasm, Exons, Genes, p53, Glioblastoma, HSP70 Heat-Shock Proteins, Hot Temperature, Humans, Mutation, Polymerase Chain Reaction, Precipitin Tests, Tumor Cells, Cultured, Tumor Suppressor Protein p53
Cancer Lett.
Date: Nov. 25, 1994
PubMed ID: 7954368
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