Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site.
Yeast cells of different cell type exhibit distinct budding patterns that reflect the organization of the actin cytoskeleton. Bud1p (Rsr1p), a Ras-like GTPase, and Bud2p, a GTPase-activating protein for Bud1p, are essential for proper budding pattern. We show that Bud2p is localized at the presumptive bud site in G(1) cells ... in all cell types and that this localization is independent of Bud1p. Bud2p subsequently localizes to the mother-bud neck after bud emergence; this localization depends on the integrity of the septins. These observations indicate that Bud2p becomes positioned in G(1) cells by recognizing cell type-specific landmarks at the presumptive bud site.
Mesh Terms:
Cell Cycle Proteins, Cell Division, Cell Polarity, Cytoskeletal Proteins, Fungal Proteins, G1 Phase, GTP Phosphohydrolase Activators, GTP Phosphohydrolases, GTPase-Activating Proteins, Microscopy, Fluorescence, Mutation, Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic, Temperature, rab GTP-Binding Proteins, ras GTPase-Activating Proteins
Cell Cycle Proteins, Cell Division, Cell Polarity, Cytoskeletal Proteins, Fungal Proteins, G1 Phase, GTP Phosphohydrolase Activators, GTP Phosphohydrolases, GTPase-Activating Proteins, Microscopy, Fluorescence, Mutation, Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Suppression, Genetic, Temperature, rab GTP-Binding Proteins, ras GTPase-Activating Proteins
Genes Dev.
Date: Aug. 01, 1999
PubMed ID: 10444589
View in: Pubmed Google Scholar
Download Curated Data For This Publication
19700
Switch View:
- Interactions 1