5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.
We have investigated the role of the RNA Polymerase II (Pol II) carboxy-terminal domain (CTD) in mRNA 5' capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, ... the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.
Mesh Terms:
Amino Acid Sequence, Animals, Binding Sites, Chloramphenicol O-Acetyltransferase, DNA-Directed RNA Polymerases, Eukaryotic Initiation Factor-4E, Globins, Glutathione Transferase, HIV-2, Humans, Mammals, Methyltransferases, Mice, Molecular Sequence Data, Nucleotidyltransferases, Peptide Initiation Factors, Phosphorylation, RNA Caps, RNA Precursors, RNA Splicing, RNA, Messenger, Recombinant Proteins, Transcription, Genetic
Amino Acid Sequence, Animals, Binding Sites, Chloramphenicol O-Acetyltransferase, DNA-Directed RNA Polymerases, Eukaryotic Initiation Factor-4E, Globins, Glutathione Transferase, HIV-2, Humans, Mammals, Methyltransferases, Mice, Molecular Sequence Data, Nucleotidyltransferases, Peptide Initiation Factors, Phosphorylation, RNA Caps, RNA Precursors, RNA Splicing, RNA, Messenger, Recombinant Proteins, Transcription, Genetic
Genes Dev.
Date: Dec. 15, 1997
PubMed ID: 9407024
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