Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit.

We report here the isolation of two genes from the yeast, Saccharomyces cerevisiae, that encode proteins closely related to mammalian p40/laminin receptor precursors (LRPs). The yeast genes, designated YST1 and YST2, encode proteins with over 95% amino acid sequence identity with one another and over 60% identity with the human ...
p40/laminin receptor precursor. The Yst/p40/37-LRP proteins are also more distantly related to the S2 family of ribosomal proteins. Analysis of the distribution of Yst1 tagged with the c-myc epitope revealed that the Yst proteins are components of the 40 S ribosomal subunit. Disruption of either YST1 or YST2 causes a significant reduction in growth rate, while disruption of both genes is lethal. Compared to wild type, polysome profiles in strains lacking either YST1 or YST2 show a pronounced shift from larger to smaller polysomes. This shift is accompanied by a substantial increase in free 60 S subunits and reduced levels of 40 S subunits. We conclude that the Yst proteins are required for translation and contribute to the assembly and/or stability of the 40 S ribosomal subunit.
Mesh Terms:
Amino Acid Sequence, Cloning, Molecular, DNA-Binding Proteins, Fungal Proteins, Gene Expression, Genes, Fungal, Humans, Introns, Molecular Sequence Data, Polyribosomes, Protein Precursors, RNA, Messenger, Receptors, Laminin, Recombinant Proteins, Ribosomal Proteins, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Sequence Tagged Sites
J. Biol. Chem.
Date: May. 10, 1996
Download Curated Data For This Publication
19734
Switch View:
  • Interactions 1