Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells.
The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. We present microarray data obtained from differentiating mouse embryonic stem cell cultures that identify an up-regulation of exocyst complex component 3-like 2 (exoc3l2) mRNA in sprouting blood vessels. Vascular expression of exoc3l2 is ... confirmed by qPCR analysis of different mouse tissues and immunofluorescence analyses of mouse brain sections. We detect an up-regulation of exoc3l2 mRNA synthesis in primary human endothelial cells in response to VEGFA, and this response is enhanced when the cells are grown on a three-dimensional collagen I matrix. Myc-tagged EXOC3L2 co-precipitates with the exocyst protein EXOC4, and immunofluorescence detection of EXOC3L2 shows partial subcellular colocalization with EXOC4 and EXOC7. Finally, we show that exoc3l2 silencing inhibits VEGF receptor 2 phosphorylation and VEGFA-directed migration of cultured endothelial cells.
Mesh Terms:
Animals, Cell Movement, Cells, Cultured, Endothelial Cells, Gene Expression Profiling, Gene Expression Regulation, Gene Silencing, Humans, Male, Mice, Oligonucleotide Array Sequence Analysis, RNA, Messenger, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-2, Vesicular Transport Proteins
Animals, Cell Movement, Cells, Cultured, Endothelial Cells, Gene Expression Profiling, Gene Expression Regulation, Gene Silencing, Humans, Male, Mice, Oligonucleotide Array Sequence Analysis, RNA, Messenger, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-2, Vesicular Transport Proteins
J. Biol. Chem.
Date: Jul. 08, 2011
PubMed ID: 21566143
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