Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly.

We have partially purified the U2 snRNP of Saccharomyces cerevisiae. Identification of some proteins consistently found in the purified fractions by nanoelectrospray mass spectrometry indicated the presence of a novel splicing factor named Rse1p. The RSE1 gene is essential and codes for a 148.2 kDa protein. We demonstrated that Rse1p ...
associates specifically with U2 snRNA at low salt concentrations. In addition, we showed that Rse1p is a component of the pre-spliceosome. Depletion of Rse1p and analysis of a conditional mutant indicated that Rse1p was required for efficient splicing in vivo. In vitro Rse1p is required for the formation of pre-spliceosomes. Database searches revealed that Rse1p is conserved in humans and that it belongs to a large protein family that includes polyadenylation factors and DNA repair proteins. The characteristics of Rse1p suggest that its human homologue could be a subunit of the SF3 splicing factor.
Mesh Terms:
Amino Acid Sequence, Animals, Conserved Sequence, DNA Repair, Fungal Proteins, Genes, Essential, Genes, Fungal, Humans, Molecular Sequence Data, Molecular Weight, Mutation, Open Reading Frames, RNA Splicing, RNA, Small Nuclear, RNA-Binding Proteins, Ribonucleoprotein, U2 Small Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Salts, Sequence Alignment, Spliceosomes, mRNA Cleavage and Polyadenylation Factors
EMBO J.
Date: Jun. 15, 1999
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