The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.
The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-alpha-factor to a site of extensive glycosylation in the Golgi apparatus in ... vitro. Two different ypt1 mutations result in loss of function of the Golgi complex without affecting the activity of the endoplasmic reticulum or soluble components required for in vitro transport. The function of the mutant Golgi apparatus can be restored by preincubation with wild-type cytosol. The transport defect observed in vitro cannot be overcome by addition of Ca++ to the reaction mixture. We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus.
Mesh Terms:
Alleles, Calcium, Carboxypeptidases, Cathepsin A, Fungal Proteins, GTP-Binding Proteins, Genes, Genes, Fungal, Glycosylation, Golgi Apparatus, Mutation, Protein Precursors, Protein Processing, Post-Translational, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Species Specificity, Vacuoles, rab GTP-Binding Proteins
Alleles, Calcium, Carboxypeptidases, Cathepsin A, Fungal Proteins, GTP-Binding Proteins, Genes, Genes, Fungal, Glycosylation, Golgi Apparatus, Mutation, Protein Precursors, Protein Processing, Post-Translational, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Species Specificity, Vacuoles, rab GTP-Binding Proteins
J. Cell Biol.
Date: Sep. 01, 1989
PubMed ID: 2504726
View in: Pubmed Google Scholar
Download Curated Data For This Publication
19901
Switch View:
- Interactions 8