Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery.
Centrins are calmodulin-like proteins that function in the duplication of microtubule-organizing centres. Here we describe a new function of the yeast centrin Cdc31. We show that overproduction of a sequence, termed CID, in the carboxy-terminal domain of the nuclear export factor Sac3 titrates Cdc31, causing a dominant-lethal phenotype and a ... block in spindle pole body (SPB) duplication. Under normal conditions, the CID motif recruits Cdc31 and Sus1 (a subunit of the SAGA transcription complex) to the Sac3-Thp1 complex, which functions in mRNA export together with specific nucleoporins at the nuclear basket. A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery.
Mesh Terms:
Active Transport, Cell Nucleus, Calcium-Binding Proteins, Cell Cycle Proteins, Fungal Proteins, Mitotic Spindle Apparatus, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Porins, Protein Binding, RNA, Messenger, RNA-Binding Proteins, Ribonucleoproteins, Saccharomyces cerevisiae Proteins
Active Transport, Cell Nucleus, Calcium-Binding Proteins, Cell Cycle Proteins, Fungal Proteins, Mitotic Spindle Apparatus, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Porins, Protein Binding, RNA, Messenger, RNA-Binding Proteins, Ribonucleoproteins, Saccharomyces cerevisiae Proteins
Nat. Cell Biol.
Date: Sep. 01, 2004
PubMed ID: 15311284
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