Interaction between the pura and E2F-1 transcription factors.
Cell proliferation is regulated by E2F-1 which facilitates expression of genes involved in entry into S-phase. Release of E2F-1 from binding partners, e.g. pRb, is critical for G1/S progression. However ectopic E2F-1 overexpression activates p53 and inhibits growth. Previously, the multifunctional Pura protein was found to bind to E2F-1 and ... inhibit E2F-1 transcriptional activity.Pura deletion mutants were assayed for: in vitro binding to E2F-1, inhibition of E2F-1-induced promoter activation and effects on cell proliferation. Two RNA species with specific binding to E2F-1 and Pura were analyzed for their effects on E2F-1/Pura binding and cell growth.The N-terminal 72 amino acids of Pura were involved in E2F-1 binding, inhibition of promoter activation by E2F-1 and reversal of E2F-mediated growth inhibition. The RNA species disrupted Pura/E2F-1 interaction and affected cell growth.E2F-1/Pura interaction has a role in the control of cell proliferation.
Mesh Terms:
Binding Sites, Cell Cycle Proteins, Cell Growth Processes, Cell Line, Tumor, Cyclic AMP Response Element-Binding Protein, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Glioblastoma, Humans, Medulloblastoma, Mutagenesis, Promoter Regions, Genetic, RNA Probes, Tetrahydrofolate Dehydrogenase, Transcription Factors, Transcriptional Activation, Transfection
Binding Sites, Cell Cycle Proteins, Cell Growth Processes, Cell Line, Tumor, Cyclic AMP Response Element-Binding Protein, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Glioblastoma, Humans, Medulloblastoma, Mutagenesis, Promoter Regions, Genetic, RNA Probes, Tetrahydrofolate Dehydrogenase, Transcription Factors, Transcriptional Activation, Transfection
Anticancer Res.
Date: Nov. 03, 2004
PubMed ID: 15517862
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