An mRNA capping enzyme targets FACT to the active gene to enhance the engagement of RNA polymerase II into transcriptional elongation.

We have recently demonstrated that an mRNA-capping enzyme, Cet1, impairs promoter proximal accumulation/pausing of RNA polymerase II (pol-II) independently of its capping activity in Saccharomyces cerevisiae to control transcription. However, it is yet unknown how pol-II pausing is regulated by Cet1. Here, we show that Cet1's N-terminal domain (NTD) promotes ...
the recruitment of FACT (FAcilitates Chromatin Transcription that enhances engagement of pol-II into transcriptional elongation) to the coding sequence of an active gene, ADH1, independently of mRNA-capping activity. Absence of Cet1's NTD decreases FACT targeting to ADH1, and consequently, reduces the engagement of pol-II into transcriptional elongation, hence leading to promoter proximal accumulation of pol-II. Similar results are also observed at other genes. Consistently, Cet1 interacts with FACT. Collectively, our results support that Cet1's NTD promotes FACT targeting to the active gene independently of mRNA-capping activity in facilitating pol-II to engage into transcriptional elongation, thus deciphering a novel regulatory pathway of gene expression.
Mol. Cell. Biol.
Date: Apr. 10, 2017
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