Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling.
Cells rely on the coordinated regulation of lipid phosphoinositides and Rab GTPases to define membrane compartment fates along distinct trafficking routes. The family of disease-related myotubularin (MTM) phosphoinositide phosphatases includes catalytically inactive members, or pseudophosphatases, with poorly understood functions. We found that Drosophila MTM pseudophosphatase Sbf coordinates both phosphatidylinositol 3-phosphate ... (PI(3)P) turnover and Rab21 GTPase activation in an endosomal pathway that controls macrophage remodeling. Sbf dynamically interacts with class II phosphatidylinositol 3-kinase and stably recruits Mtm to promote turnover of a PI(3)P subpool essential for endosomal trafficking. Sbf also functions as a guanine nucleotide exchange factor that promotes Rab21 GTPase activation associated with PI(3)P endosomes. Of importance, Sbf, Mtm, and Rab21 function together, along with Rab11-mediated endosomal trafficking, to control macrophage protrusion formation. This identifies Sbf as a critical coordinator of PI(3)P and Rab21 regulation, which specifies an endosomal pathway and cortical control.
Mesh Terms:
Animals, Cell Membrane, Cells, Cultured, Drosophila, Drosophila Proteins, Endosomes, Guanine Nucleotide Exchange Factors, Macrophages, Phosphatidylinositol Phosphates, Protein Transport, Protein Tyrosine Phosphatases, Non-Receptor, RNA Interference, RNA, Small Interfering, rab GTP-Binding Proteins
Animals, Cell Membrane, Cells, Cultured, Drosophila, Drosophila Proteins, Endosomes, Guanine Nucleotide Exchange Factors, Macrophages, Phosphatidylinositol Phosphates, Protein Transport, Protein Tyrosine Phosphatases, Non-Receptor, RNA Interference, RNA, Small Interfering, rab GTP-Binding Proteins
Mol. Biol. Cell
Date: Jul. 01, 2012
PubMed ID: 22648168
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