Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions.

Phosphorylation of the human histone variant H2A.X and H2Av, its homolog in Drosophila melanogaster, occurs rapidly at sites of DNA double-strand breaks. Little is known about the function of this phosphorylation or its removal during DNA repair. Here, we demonstrate that the Drosophila Tip60 (dTip60) chromatin-remodeling complex acetylates nucleosomal phospho-H2Av ...
and exchanges it with an unmodified H2Av. Both the histone acetyltransferase dTip60 as well as the adenosine triphosphatase Domino/p400 catalyze the exchange of phospho-H2Av. Thus, these data reveal a previously unknown mechanism for selective histone exchange that uses the concerted action of two distinct chromatin-remodeling enzymes within the same multiprotein complex.
Mesh Terms:
Acetyl Coenzyme A, Acetylation, Acetyltransferases, Adenosine Triphosphatases, Animals, Cell Line, DNA Damage, DNA Repair, Dimerization, Drosophila Proteins, Drosophila melanogaster, Embryo, Nonmammalian, Histone Acetyltransferases, Histones, Multiprotein Complexes, Nucleosomes, Phosphorylation, RNA Interference, Recombinant Proteins, Transcription Factors
Science
Date: Dec. 17, 2004
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