Drosophila hold'em is required for a subset of meiotic crossovers and interacts with the dna repair endonuclease complex subunits MEI-9 and ERCC1.

Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to ...
the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.
Mesh Terms:
Amino Acid Sequence, Animals, Chromosome Mapping, Crossing Over, Genetic, DNA Breaks, Double-Stranded, DNA Repair, DNA-Binding Proteins, Drosophila Proteins, Drosophila melanogaster, Meiosis, Methyl Methanesulfonate, Molecular Sequence Data, Mutagens, Mutation, Nondisjunction, Genetic, Nuclear Proteins, Protein Binding, Protein Subunits, Two-Hybrid System Techniques
Date: Jan. 01, 2009
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