Mutational analysis of the Drosophila DNA repair and recombination gene mei-9.
Drosophila mei-9 is essential for several DNA repair and recombination pathways, including nucleotide excision repair (NER), interstrand crosslink repair, and meiotic recombination. To better understand the role of MEI-9 in these processes, we characterized 10 unique mutant alleles of mei-9. These include a P-element insertion that disrupts repair functions but ... not the meiotic function; three nonsense mutations, one of which has nearly wild-type levels of protein; three missense mutations, one of which disrupts the meiotic function but not repair functions; two small in-frame deletions; and one frameshift.
Mesh Terms:
Alleles, Amino Acid Motifs, Amino Acid Sequence, Animals, Blotting, Western, Codon, Nonsense, DNA Damage, DNA Mutational Analysis, DNA Repair, Drosophila Proteins, Drosophila melanogaster, Frameshift Mutation, Gene Deletion, Meiosis, Models, Genetic, Molecular Sequence Data, Mutation, Mutation, Missense, Nuclear Proteins, Recombination, Genetic, Sequence Homology, Amino Acid, Two-Hybrid System Techniques, Ultraviolet Rays
Alleles, Amino Acid Motifs, Amino Acid Sequence, Animals, Blotting, Western, Codon, Nonsense, DNA Damage, DNA Mutational Analysis, DNA Repair, Drosophila Proteins, Drosophila melanogaster, Frameshift Mutation, Gene Deletion, Meiosis, Models, Genetic, Molecular Sequence Data, Mutation, Mutation, Missense, Nuclear Proteins, Recombination, Genetic, Sequence Homology, Amino Acid, Two-Hybrid System Techniques, Ultraviolet Rays
Genetics
Date: May. 01, 2004
PubMed ID: 15166153
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