A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80.
The GAL4 activator and GAL80 repressor proteins regulate the expression of yeast genes in response to galactose. A complex of the two proteins isolated from glucose-grown cells is inactive in an in vitro transcription reaction but binds DNA and blocks activation by the GAL4-VP16 chimeric activator. The complex purified from ... galactose-grown cells contains a mixture of phosphorylated and unphosphorylated forms of GAL4. The galactose-induced form of GAL4 activates in vitro transcription to levels similar to those seen with GAL4-VP16. The induced GAL4 complex is indistinguishable in size and apparent shape from the uninduced complex, consistent with a continued association with GAL80. These results confirm in vivo analyses that correlate GAL4 phosphorylation with galactose induction and support a model of transcriptional activation that does not require GAL80 dissociation.
Mesh Terms:
Base Sequence, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Molecular Sequence Data, Phosphorylation, RNA Polymerase II, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
Base Sequence, DNA, Fungal, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Molecular Sequence Data, Phosphorylation, RNA Polymerase II, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
Mol. Cell. Biol.
Date: Nov. 01, 1992
PubMed ID: 1406674
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