Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3.
DNA fragmentation is the hallmark of apoptotic cells and mainly mediated by the DNA fragmentation factor DFF40(CAD)/DFF45(ICAD). DFF40 is a novel nuclease, whereas DFF45 is an inhibitor that can suppress the nuclease activity. Apoptotic DNA fragmentation in the fly is controlled by four DFF-related proteins, known as Drep1, 2, 3 ... and 4. However, the functions of Drep2 and Drep3 are totally unknown. Here, we found that Drep2 is a novel nuclease whose activity is inhibited by Drep3 through a tight interaction with the CIDE domain. Our results suggest that the fly has dual apoptotic DNA fragmentation systems: Drep1: Drep4 and Drep2: Drep3 complexes.
Mesh Terms:
Amino Acid Sequence, Animals, Apoptosis, Apoptosis Regulatory Proteins, Caspase 1, DNA Fragmentation, Deoxyribonucleases, Drosophila Proteins, Drosophila melanogaster, Molecular Sequence Data, Protein Interaction Domains and Motifs, Recombinant Proteins
Amino Acid Sequence, Animals, Apoptosis, Apoptosis Regulatory Proteins, Caspase 1, DNA Fragmentation, Deoxyribonucleases, Drosophila Proteins, Drosophila melanogaster, Molecular Sequence Data, Protein Interaction Domains and Motifs, Recombinant Proteins
FEBS Lett.
Date: Sep. 21, 2012
PubMed ID: 22850116
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