Structure of PCNA from Drosophila melanogaster.
Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The crystal of DmPCNA diffracted to 2.0 A resolution and belonged to space group H3, with unit-cell parameters a = b = ... 151.16, c = 38.28 A. The structure of DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links the N- and C-terminal domains. Additionally, the N-terminal and C-terminal domains contact each other through hydrophobic associations. Compared with human PCNA, the IDCL of DmPCNA has conformational changes, which may explain their difference in function. This work provides a structural basis for further functional and evolutionary studies of PCNA.
Mesh Terms:
Amino Acid Sequence, Animals, Drosophila melanogaster, Humans, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Molecular Sequence Data, Proliferating Cell Nuclear Antigen, Protein Structure, Quaternary, Protein Structure, Tertiary, Sequence Alignment
Amino Acid Sequence, Animals, Drosophila melanogaster, Humans, Hydrophobic and Hydrophilic Interactions, Models, Molecular, Molecular Sequence Data, Proliferating Cell Nuclear Antigen, Protein Structure, Quaternary, Protein Structure, Tertiary, Sequence Alignment
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Date: Apr. 01, 2013
PubMed ID: 23545643
View in: Pubmed Google Scholar
Download Curated Data For This Publication
203698
Switch View:
- Interactions 1