RNA binding and translational suppression by bicoid.
The anterior determinant bicoid (bcd) of Drosophila is a homeodomain protein. It forms an anterior-to-posterior gradient in the embryo and activates, in a concentration-dependent manner, several zygotic segmentation genes during blastoderm formation. Its posterior counterpart, the homeodomain transcription factor caudal (cad), forms a concentration gradient in the opposite direction, emanating ... from evenly distributed messenger RNA in the egg. In embryos lacking bcd activity as a result of mutation, the cad gradient fails to form and cad becomes evenly distributed throughout the embryo. This suggests that bcd may act in the region-specific control of cad mRNA translation. Here we report that bcd binds through its homeodomain to cad mRNA in vitro, and exerts translational control through a bcd-binding region of cad mRNA.
Mesh Terms:
Animals, Base Sequence, Binding Sites, DNA Primers, Diptera, Drosophila, Drosophila Proteins, Female, Gene Expression Regulation, Homeodomain Proteins, Insect Hormones, Molecular Sequence Data, Protein Binding, Protein Biosynthesis, RNA, Messenger, RNA-Binding Proteins, Recombinant Fusion Proteins, Trans-Activators, Transcription Factors, Transfection
Animals, Base Sequence, Binding Sites, DNA Primers, Diptera, Drosophila, Drosophila Proteins, Female, Gene Expression Regulation, Homeodomain Proteins, Insect Hormones, Molecular Sequence Data, Protein Binding, Protein Biosynthesis, RNA, Messenger, RNA-Binding Proteins, Recombinant Fusion Proteins, Trans-Activators, Transcription Factors, Transfection
Nature
Date: Feb. 22, 1996
PubMed ID: 8602224
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