The yeast nuclear pore complex: composition, architecture, and transport mechanism.
An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify ... all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport.
Mesh Terms:
Biological Transport, Carrier Proteins, Chromatography, High Pressure Liquid, Fluorescent Antibody Technique, Fungal Proteins, Membrane Proteins, Microscopy, Immunoelectron, Models, Biological, Molecular Weight, Nuclear Envelope, Nuclear Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Biological Transport, Carrier Proteins, Chromatography, High Pressure Liquid, Fluorescent Antibody Technique, Fungal Proteins, Membrane Proteins, Microscopy, Immunoelectron, Models, Biological, Molecular Weight, Nuclear Envelope, Nuclear Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
J. Cell Biol.
Date: Feb. 21, 2000
PubMed ID: 10684247
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