The SAGA complex, together with transcription factors and the endocytic protein Rvs167p, coordinate the reprofiling of gene expression in response to changes in sterol composition in Saccharomyces cerevisiae.

Changes in cellular sterol species and concentrations can have profound effects on the transcriptional profile. In yeast, mutants defective in sterol biosynthesis show a wide range of changes in transcription, including a co-induction of anaerobic genes and ergosterol biosynthesis genes, biosynthesis of basic amino acids, and several stress genes. However ...
the mechanisms underlying these changes are unknown. We identified mutations in the SAGA complex, a co-activator of transcription, which abrogate the ability to carry out most of these sterol-dependent transcriptional changes. In the erg3 mutant, the SAGA complex increases its occupancy time on many of the induced ergosterol and anaerobic gene promotors, increases its association with several relevant transcription factors, the SWI/SNF chromatin remodeling complex and surprisingly, associates with an endocytic protein, Rvs167p, suggesting a moonlighting function for this protein in the sterol-regulated induction of the heat shock protein, HSP42 and HSP102, mRNAs.
Mol. Biol. Cell
Date: Aug. 02, 2017
Download Curated Data For This Publication
204936
Switch View:
  • Interactions 16