Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.

Fission yeast cells use Arp2/3 complex and formin to assemble diverse filamentous actin (F-actin) networks within a common cytoplasm for endocytosis, division, and polarization. Although these homeostatic F-actin networks are usually investigated separately, competition for a limited pool of actin monomers (G-actin) helps to regulate their size and density. However, ...
the mechanism by which G-actin is correctly distributed between rival F-actin networks is not clear. Using a combination of cell biological approaches and in vitro reconstitution of competition between actin assembly factors, we found that the small G-actin binding protein profilin directly inhibits Arp2/3 complex-mediated actin assembly. Profilin is therefore required for formin to compete effectively with excess Arp2/3 complex for limited G-actin and to assemble F-actin for contractile ring formation in dividing cells.
Mesh Terms:
Actin Cytoskeleton, Actin-Related Protein 2-3 Complex, Actins, Endocytosis, Homeostasis, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Profilins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins
Dev. Cell
Date: Jan. 12, 2015
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